The first OuestOÏD conference was held in Rennes on April 4, with around a hundred participants. On the program for the day dedicated to organoids and 3D models, scientific presentations by high-level researchers and presentations of innovative methodologies for 3D models by technological core facilities.

                             

 

Alexandre Baffet de the Curie Institute (Paris) opened the day with a keynote reading of his research work on understanding the development of the neocortex, and in particular the fate of progenitor cells (multiplication/differentiation) using models of cerebral organoids and correlative microscopy analyzes .

(see the latest publication on this work: Coquand L, et al. A cell fate decision map reveals abundant direct neurogenesis bypassing intermediate progenitors in the human developing neocortex. Nat Cell Biol. 2024 Mar 28. doi: 10.1038/s41556-024-01393 -z. Epub ahead of print. PMID: 38548890).

 

Bertrand Pain, Stem Cell and Brain Research Institute in Lyon, continued this first session dedicated to cerebral organoids, with a communication on the evaluation of the effects of tramadol, an opioid analgesic using cortical organoids

 

After a first break dedicated to posters, the second session allowed participants to discover the activities of the core facility Orgapred of Caen led by Laurent Poulain, which develops patient-derived tumor organoid (PDTO) models for the evaluation of therapeutic solutions or predictive tools.

 

A series of short oral communications followed, with interventions from students, post-docs and researchers from the OuestOÏD network:

  • Veranika Panasenkava de the Institute of Genetics and Development of Rennes presented a model of human neurectoderm development vitro based on iPSC cells, as part of the search for new molecular diagnostic tools for holoprosencephaly (HPE), a rare disease of brain development (see his poster https://uncloud.univ-nantes.fr/index.php/s/dYTsTPndGW635zD)
  • Allwynn Pereira of the Institute Target de Nantes described the establishment of microglial cells from iPSC cells, with the aim of developing complex models of brain organoids and retina.
  • Lucille Moutaux of the institute Numecan from Rennes, presented the development and phenotypic characterization of an organoid model derived from a patient suffering from hepatic cancer, to validate metabolic targets.
  • Marion Desdouits of the research unit MASAE (Microbiology, Food, Health, Environment) from Nantes concluded the morning with a communication on the use of intestinal organoid models to evaluate the infection capabilities of enteric viruses that have contaminated coastal shellfish.

After the lunch break, Stefan Giselbrecht du MERLN Institute from Maastricht gave the second keynote reading of the day, and detailed his “morphogenetic micro-engineering” approach, which uses stem cells cultured on tailor-made modular “bio-reactors”, which can be exposed to dynamic environmental conditions. and controlled using microfluidics, and allowing easier and more efficient access to multi-omics analyzes downstream of 3D culture.

Jean-Baptiste Dupont, from the institute TARGET from Nantes, continued this “engineering” session with the presentation of a muscle tissue model (EMT) based on human iPS cells and fibrin hydrogels, used for the study of Duchenne muscular dystrophy and the development new gene therapies based on AAV vectors.

Two short communications followed:

  • Julie Warin, of the group BIODIV from RMeS in Nantes described its work to develop and validate optimal conditions for culturing iPSC cells to obtain their maturation into notochordal cells and their terminal differentiation into adult intervertebral disc cells.
  • Jean-Jacques Lareyre du LPGP from Rennes showed the establishment of a Zebrafish testicular organoid model, as well as its characterization by 3D imaging.

After a final break, the last session of the day involved Nicolas Lebonvallet du Laboratory Interactions Epithelia Neurons of Brest, on the morphological and functional description of a model of skin innervated by sensory neurons

Patrice Hémon continued this session with the presentation of the Hyperion core facility in Brest, with the description of the Mass Cytometry Imaging methodology, its possibilities, and its application for the imaging of 3D models with the example of visualization of the model of innervated skin previously described by Nicolas Lebonvallet.

 

Jean-Luc Galzi, Department of Biotechnology and Cell Signaling d'Illkirch, closed the day by addressing issues of ethics and scientific integrity with the presentation of the Hybrida project. The project, funded by Europe's Horizon 2020 program, develops tools available to the scientific community: the MIAOU (Minimal Information About an Organoid and its Use) useful for exchanges between researchers, the EChOES (Evaluator Checklist for Organoid Ethical Studies) for exchanges between researchers and evaluators. For more information, visit the Hybrida website (http://hybridaogl.eu/).

The day concluded with an assessment of the OuestOÏD unifying project and the perspectives of the network by Lawrence David (CR2TI/iPSC, Nantes) and Yannick Arlot (IGDR/Biosit, Reindeer).

                                                                                 

Thanks !

  • to our moderators:

Gaëlle Friocourt and Joël Eyer

Anne Camus and Aurélien Bidaud-Meynard

Franck Halary and Laurent Griscom

Yannick Arlot and Laurent David

 

  • to our organizing committee:

Bénédicte Martin

Yannick Arlot

Lawrence David

Aurélien Bidaud-Meynard

Nathalie Melaine

Jocelyne Le Seyec

Laurent Griscom

Sandrine Lombard

  • to the Bretagne and Pays de la Loire regions who financed the OuestOÏD project,
  • to all partner SFRs of the OuestOÏD network (Bonamy, Biosit, Ibsam, iCAT).

 

photo credits: Biogenouest animation unit, Laurent Griscom BIM3D

Post Views: 13