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As part of Biogenouest's bio-imaging axis, the H2P2, MRic, APEX and MicroPICell core facilities offer you a day of animation around transparency of samples for microscopy. The speakers will talk about their feedback on the different transparency methods, their acquisition method and the results obtained. Discussion times will allow you to submit your issues or provide your own solutions.

Program :

09:30 Introduction of the day
Transparency and imaging of samples on MicroPICell, by Marine Malloci, Nantes.
Transparency, immunolabeling and visualization of embryonic hearts and heart valves in rats by Pascal Aumond, Institut du Thorax, Nantes.
Discussion

10:15 Transparency and imaging of samples on MRic by Xavier Pinson, Rennes.
“Transparency of secondary lymphoid organs in humans” by Frédéric Mourcin, MICMAC, Rennes.
“Transparization tests on Xenopus eye and on spheroids from crystalline epithelial cells” by David Reboutier, IGDR, Rennes.
Discussion

11:00 Break

11:30 Transparency and imaging of samples on APEX by Laurence Dubreil, Nantes.

"Transparency of tomatoes by Rapiclear and / or CUBIC to analyze the structure of the cuticle via the second harmonic signals generated by crystalline cellulose" by Nathalie Geneix, Ellipse team, BIA INRAE, Nantes. "Multi-labeling on pig lymph node and transparency by the iDISCO method ”by Mireille Ledevin and Laurence Dubreil, APEX PAnTher INRAE ​​Oniris, in collaboration with Nicolas Bertho, UMR BioE by INRAE ​​Oniris, Nantes.

12:20 Transparency and imaging of samples on H2P2, by Nicolas Mouchet Rennes.
“Image analysis: services offered by core facilities and tools adapted to thick samples” by Thierry Pécot and Magalie Feyeux
Discussion

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