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Technology Cell DIVE from the company Leica offers an in-depth solution of the characterization of tissue microenvironment using multiplexed imaging technology.

The privileged technical and commercial relations between the histopathology core facility and the laboratory equipment manufacturer have accelerated a unique collaboration in Europe. Indeed, for several weeks an integral solution of the Cell DIVE is in Rennes at the premises of H2P2 so that Alain Fautrel and Nicolas Mouchet's team can experience the full potential of this technology in a situation.

For example, in cancer treatment research, researchers need a better understanding of the cellular architecture of normal and diseased tissues to develop better treatments and more accurately predict disease progression. Thus, multiplexed or hyperplex imaging is the latest technology that clearly visualizes, identifies and quantifies important biomarkers. The multiplexed imaging solution Cell DIVE allows spatial analysis of biomarkers. It provides thousands of data points from a single section of fabric, down to the level of a single cell.

customizable : Design and configure experiences exactly the way you want them.
Specific : Collect the data that matters to you and trust your results.
Proven : years of experience plus three tested and refined technologies, merged into one solution.

The H2P2 core facility team is available to discuss your project by mobilizing this unique equipment in Europe.

David Pointu, from Leica, presents the equipment in place at H2P2 in the video below.

H2P2 - European core facility Cell DIVE multiplex technology

Cell DIVE technology makes it possible to visualize tissue sections 5 µm thick up to 60 proteins. The principle consists in carrying out successive immunolabeling of 4 antibodies conjugated with 4 fluorochromes Cy2, CY3, Cy5 and Cy7, the slides are digitized as and compiled. The data is then processed via the HALO software for the identification of clusters, Heatmap neighborhood analysis, etc.
On the strength of our experience in immunofluorescence, we are going to optimize the markings for the benefit of the Rennes research teams and soon we intend to welcome projects at European level and to share our experience of this innovative, non-destructive and innovative technology with other laboratories. inexpensive (no secondary antibody, no kit, etc.).