H2P2 - European core facility Cell DIVE multiplex technology
Cell DIVE technology makes it possible to visualize tissue sections 5 µm thick up to 60 proteins. The principle consists in carrying out successive immunolabeling of 4 antibodies conjugated with 4 fluorochromes Cy2, CY3, Cy5 and Cy7, the slides are digitized as and compiled. The data is then processed via the HALO software for the identification of clusters, Heatmap neighborhood analysis, etc.
On the strength of our experience in immunofluorescence, we are going to optimize the markings for the benefit of the Rennes research teams and soon we intend to welcome projects at European level and to share our experience of this innovative, non-destructive and innovative technology with other laboratories. inexpensive (no secondary antibody, no kit, etc.).