Electron microscopy in life sciences, exploration from molecule to tissue in the fields of health, biomaterials, food sciences.
Electron microscopy is a technology in strong development within the scientific community of the Great West. This day will aim to bring together scientists, core facility managers and future users of this technology around presentations that will cover life science applications in the fields of health, biomaterials, food.
The event was held face-to-face on the site ofOniris – Food Science Campus à Nantes.
8: 50 | Introduction of the day (Laurence DUBREIL, Philippe MOREAU)
Session 1: Moderators: Angélina D'ORLANDO (BIBS) and Romain MALLET (SCIAM)
9: 00 | “Cell walls: their variability in the grain and their impact on wheat quality” by Fabienne GUILLON, UR 1268 BIA, Nantes
9: 30 | “Lactic acidosis and mitochondrial dysfunction in macrophages – the contribution of electron microscopy” by Léa PAOLINI, UMR 1307 CRCI2NA, Team 4, Angers
10:00 | (not available in replay) "Cryo-SEM observation of cells in a highly hydrated hydrogel, cryofixed in pasty nitrogen" by Sébastien GRASTILLEUR, SC3M/UMR 1229 RMeS, Nantes
10: 30 | Break
Session 2: Moderators: Joëlle VEZIERS (SC3M) and Philippe MOREAU (IMN)
11: 00 | "Characterization of the structural heterogeneity of microtubules by electronic cryo-tomography" by Denis CHRETIEN, MRic, Rennes
11: 30 | “From macroscopic fiber to nanofiber: visualization of cellulose at different scales by electron microscopy” by Ana VILLARES, UR 1268 BIA, Nantes
12: 00 | “3D SEM: serial block face Imaging by in situ ultramicrotomy” by Etienne GONTIER, Bordeaux Imaging Center, Bordeaux
12: 30 | Buffet
Session 3: Moderators: Laurence DUBREIL (APEX) and Bruno NOVALES (BIBS)
13: 30 |"Preparation of cellular samples for electron microscopy and chemical imaging" by Benoit GALLET, UMR 5075 Institut de Biologie Structurale, Grenoble
14:00 | (not available in replay) "Elucidating the solid-liquid interface of (bio)materials using 3D-FIB/SEM and STEM/EELS at cryogenic temperatures" by Patricia ABELLAN, UMR 6502 IMN, Nantes
14: 30 | “Electron microscopy for studying V0-ATPase function in brush border maintenance and related absorption disorders: combined use of C. elegans and intestinal organoids” by Ophélie NICOLLE, UMR 6061 IGDR, Rennes
15: 00 | "Examples of S/TEM Nant'Themis applications in life sciences" by Eric GAUTRON, UMR 6502 IMN, Nantes
15: 30 | “Extracellular vesicles: from tissue observation to 3D modeling after electronic cryo-tomography” by Aurélien DUPONT, MRic, Rennes
16: 00 | “Image analysis methods for correlative microscopy” by Perrine PAUL-GILLOTEAUX, MicroPICell, Nantes
16: 30 | Conclusion